Understand that no actual insects are involved; it's a metaphor for internal jitteriness or unease. During the long meeting, I really had ants in my pants waiting for my turn to speak.
Pro Tip 2/3
Informal Usage
It's conversational and best used in casual settings, not formal or academic ones. Sorry for squirming, I just have ants in my pants today!
Pro Tip 3/3
Avoid Overuse
Using this phrase too often might confuse non-native speakers or seem unprofessional. I know I said he has ants in his pants, but I mean he's just really energetic.