Chloroform/ˈklɔːrəfɔːrm/ Definition 1 of 2Pro Tip 1/3Medical Use Chloroform was historically used for anesthesia in surgeries but is less common now. Chloroform isn't the first choice for anesthesia these days. Pro Tip 2/3Dangerous Chemical Chloroform is toxic and poses health risks, which limits its use. Handle chloroform with care to avoid poisoning. Pro Tip 3/3Science Labs In a lab, chloroform is used to extract substances and in chemical reactions. Add chloroform to separate the compounds.