Use 'ladyship' respectfully when speaking to or about noblewomen, or sarcastically to mock someone acting superior. As you wish, your ladyship.
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Historical Use
'Ladyship' was more common in the past and might appear frequently in historical or period pieces. The novel depicted the servant addressing the countess as 'her ladyship'.
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Cultural Nuance
Being aware of cultural norms is crucial, as incorrectly addressing someone as 'ladyship' might be seen as mockery. In the modern office, calling a superior 'your ladyship' could be seen as sarcastic.