Amen/ɑːˈmen/ Definition 1 of 3Pro Tip 1/3Prayer Conclusion Used traditionally to finish Christian prayers, signifying consent and reverence. After the prayer, we bowed our heads and softly spoke 'amen.' Pro Tip 2/3Group Agreement In group settings, 'amen' represents collective agreement or affirmations. When the preacher finished, the congregation echoed with a single 'amen.' Pro Tip 3/3Cultural Variance 'Amen' may be pronounced differently across Christian denominations and cultures. In her church, they pronounced it 'ah-meen' instead of 'ay-men.'