Use 'mark you' to emphasize a trait that contrasts with what was stated just before. He's very playful, mark you, but he's also incredibly responsible.
Pro Tip 2/3
Unexpected Qualifier
'Mark you' introduces a point that may surprise the listener, providing a twist to the sentence. This painting is quite ordinary, mark you, but it's worth a fortune.
Pro Tip 3/3
Adding Perspective
Use it to provide additional information that gives perspective or balance to a statement. They arrived late, mark you, due to unforeseen traffic.